The silica-based extraction method is the industry standard. DNA binds to silica in the presence of high concentrations of chaotic salts, allowing impurities to be washed away before the DNA is eluted into a clean buffer. 4. Library Preparation and Sequencing
Modern DNA from researchers or the environment is "fresher" and more intact than aDNA, making it easy for a tiny amount of modern DNA to overwhelm the ancient sample. 2. Sample Selection and Preparation Ancient DNA: Methods and Protocols
Proteinase K is added to break down cellular proteins and nucleases. The silica-based extraction method is the industry standard