Set a limit in gigabytes (e.g., -m 8 for 8GB).
It reduces the number of mismatches and short indels (insertions/deletions) by running a post-processing tool called MismatchCorrector. SPADES-Day2-pc.rar
If you are following a standard microbial genomics curriculum, your "Day 2" workflow typically involves: Set a limit in gigabytes (e
Using tools like Trimmomatic to clean your raw data before assembly. Set a limit in gigabytes (e.g.
When running SPAdes (the executable often found in such packages as spades.py ), the --careful option is a critical feature for small genome projects (like bacteria, which are common in workshops).